Work

E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel

Year: 2010

Type: article

Abstract: E-p-methoxycinnamic acid (MCA) is one of the main active constituents of Scrophularia buergeriana Miquel and has hepatoprotective, anti-amnestic, and neuroprotective activities. For studying in vitro production of MCA, we established a hairy root culture of S. buergeriana by infecting leaf explants with Agrobacterium rhizogenes R1000, and tested the growth and MCA production of these cultures using different strengths of media and concentrations of auxins. Hairy roots grown in half-strength MS medium showed the highest levels of growth (10.3 g/l) as well as MCA production (0.83 mg/100 g dry weight). Hairy root culture with the treatment of 0.5 mg/l IBA produced the highest amount of dry weight (11.8 g/l) and MCA (1.26 mg/100 g dry weight) production. These results demonstrate that the hairy root culture of S. buergeriana is a valuable alternative approach for the production of MCA. .

Source: Archives of Biological Sciences

DOI: 10.2298/abs1003649p

Authors: Sang Park, Xiaohua Li, Seok Eom, Chung Lee, Sook Lee

Institutions: Chungnam National University, Kyung Hee University, Pusan National University, Chosun University

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

Funder: Korea Science and Engineering Foundation