Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus
Year: 1997
Type: article
Abstract: The finding of heat-stable enzymes or the engineering of moderately thermostable enzymes into more stable ones by random or site-directed mutagenesis has become a main priority of modern biotechnology. We report here for the first time a heat-stable phytase able to withstand temperatures up to 100 degrees C over a period of 20 min, with a loss of only 10% of the initial enzymatic activity. The gene (phyA) encoding this heat-stable enzyme has been cloned from Aspergillus fumigatus and overexpressed in Aspergillus niger. The enzyme showed high activity with 4-nitrophenyl phosphate at a pH range of 3 to 5 and with phytic acid at a pH range of 2.5 to 7.5.
Authors: L Pasamontes, M Haiker, M Wyss, M Tessier, A P van Loon
Institution: F. Hoffmann-La Roche AG
Language: English
Topic: Phytase and its Applications
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2168848023