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Probing the Spatial Dependence of the Emission Spectrum of Single Human Retinal Lipofuscin Granules Using Near-field Scanning Optical Microscopy¶

Year: 2001

Type: article

Abstract: The emission spectra of single lipofuscin granules are examined using spectrally resolved confocal microscopy and near-field scanning optical microscopy (NSOM). The emission spectrum varies among the granules examined revealing that individual granules are characterized by different distributions of fluorophores. The range of spectra observed is consistent with in vivo spectra of human retinal pigment epithelium cells. NSOM measurements reveal that the shape of the spectrum does not vary with position within the emissive regions of single lipofuscin granules. These results suggest that the relative distribution of fluorophores within the emissive regions of an individual granule is homogeneous on the spatial scale approximately 150 nm.

Source: Photochemistry and Photobiology

DOI: 10.1562/0031-8655(2001)0740364ptsdot2.0.co2

Authors: Nicole M. Haralampus-Grynaviski, Laura E. Lamb, John D. Simon, Jeffrey R. Krogmeier, Robert C. Dunn, Anna Pawlak, Malgorzata Rózanowska, Tadeusz Sarna, Janice M. Burke

Institutions: Duke University, Duke Medical Center, University of Kansas, Jagiellonian University, Medical College of Wisconsin

Language: English

Topic: Near-Field Optical Microscopy

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2169260237

FWCI: 1.908

Cites: 40

Cited by: 22

Related to: 10