Work

A PCR-oligonucleotide ligation assay to determine the prevalence of 23S rRNA gene mutations in clarithromycin-resistant Helicobacter pylori

Year: 1997

Type: article

Abstract: We have developed a rapid PCR-oligonucleotide ligation assay that can discriminate single base substitutions that are associated with clarithromycin resistance in Helicobacter pylori. Susceptible isolates were wild type at positions 2143 and 2144 (cognate to 2058 and 2059 in Escherichia coli), while 93% of the resistant isolates contained A-to-G mutations at either position and 7% of the isolates contained A-to-C mutations at position 2143. In addition, the MIC for 86% of the resistant isolates with an A2143 mutation was > or = 64 micrograms per ml, and that for 89% of the resistant isolates with an A2144 mutation was < or = 32 micrograms per ml.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.41.3.712

Authors: G G Stone, D Shortridge, J Versalovic, J Beyer, R K Flamm, D Y Graham, A T Ghoneim, S K Tanaka

Institution: Abbott (United States)

Language: English

Topic: Helicobacter pylori-related gastroenterology studies

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2169501912

FWCI: 15.33

Cites: 16

Cited by: 162

Related to: 10