Year: 2015
Type: article
Abstract: A survey of non-cultivated plants of the Tallgrass Prairie Preserve of Oklahoma for viruses used young leaf tissue for analysis of virus sequence signature content.To assess whether tissue selection may affect the results of such a survey, Snow-on-the-mountain (Euphorbia marginata Pursh) plants growing along roadsides of Payne Co., Oklahoma, were sampled during the fall of 2007.Specimens of upper leaves, lower leaves, white foliar bracts and fruits were separately processed to obtain nucleic acid preparations from virus-like particle fractions of plant homogenates.Signatures of two viruses, a comovirus and a tymovirus, Asclepias asymptomatic virus (AsAV), both also found on the Tallgrass Prairie Preserve, were observed in the sequences of the nucleic acid preparations.Not all tissues of positive plants were consistently positive.In the sequences retrieved, the nucleotides at only five positions differed from those of AsAV obtained from Asclepias viridis.Sequences from bracts and from lower leaves of different plants were more similar to one another than to other sequences from the same plant.
Author: Jennifer Hackett
Language: English
Topic: Plant Virus Research Studies
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2169592145