The hrp genes of Pseudomonas cichorii are essential for pathogenicity on eggplant but not on lettuce
Year: 2008
Type: article
Abstract: Pseudomonas cichorii causes necrotic lesions in eggplant and rot in lettuce. Through transposon insertion into P. cichorii strain SPC9018 we produced two mutants, 4-57 and 2-99, that lost virulence on eggplant but not lettuce. Analyses showed that a transposon was inserted into the hrpG gene in 4-57 and the hrcT gene in 2-99. Nucleotide sequences of the hrp genes of SPC9018 are homologous to those of Pseudomonas viridiflava BS group strains. The pathogenicity of 4-57 on eggplant was restored by transformation with an hrpF operon, originating from either SPC9018 or the BS group member P. viridiflava strain 9504 (Pv9504). These data suggested the involvement of hrp genes in the pathogenicity of SPC9018 on eggplant, and functional conservation of hrpF operons between SPC9018 and Pv9504. Both the hrpS mutant and the hrpL mutant were unable to cause necrotic lesions on eggplant leaves but retained their pathogenicity against lettuce. These results suggest that the pathogenicity of P. cichorii is hrp-dependent in eggplant, but not in lettuce.
Source: Microbiology
Authors: Hiroshi Hojo, Makoto Koyanagi, Masayuki Tanaka, Shigeru Kajihara, Kouhei Ohnishi, Akinori Kiba, Yasufumi Hikichi
Institutions: University of Kochi, Kōchi University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
Awards: Studies on mechanism of Ralstoniia solanacearum proliferation in host plants - Comprehensive analysis of interaction between R.solanacearum and host plants -, Exploration of the sequential fundamental processes in the early infection stage of Ralstonra solanacearum, 植物細菌病をモデルに植物の罹病化・発病の分子機構を解明する。, 植物の自己細胞死の制御による植物細菌病の防除への展望, Evolutional and functional studies on diversification of virulence genes of plant bacteria with establishment of host species
OpenAlex ID: W2169605202