Work

Optimization of the Pyridyl Nucleobase Scaffold for Polymerase Recognition and Unnatural Base Pair Replication

Year: 2008

Type: article

Abstract: That's not natural! Unnatural base pairs formed between pyridyl-nucleotides have been shown to be promising candidates for the expansion of the genetic alphabet. We now demonstrate that the pyridyl nucleobase scaffold can be optimized for replication, both as a self pair and as a component of a heteropair. As a result, we have identified a pyridyl-nucleotide self pair that is well recognized by a DNA polymerase, allowing it to be replicated and used to synthesize site-specifically labeled DNA in good yields.

Source: ChemBioChem

DOI: 10.1002/cbic.200800577

Authors: Yoshiyuki Hari, Gil Tae Hwang, Aaron M. Leconte, Nicolas Joubert, Michal Hocek, Floyd E. Romesberg

Institutions: Scripps Research Institute, Czech Academy of Sciences, Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G1387825320, Expanding the Genetic Alphabet by Design and Selection