Work

The Linker Region of NS3 Plays a Critical Role in the Replication and Infectivity of Hepatitis C Virus

Year: 2014

Type: article

Abstract: Hepatitis C virus (HCV) NS3-4A is required for viral replication and assembly. We establish that virus assembly is sensitive to mutations in the linker region between the helicase and protease domains of NS3-4A. However, we find that the protease cleavage, RNA binding, and unwinding rates of NS3 are minimally affected in vitro. Thus, we conclude that the NS3 linker is critical for mediating protein-protein interactions and dynamic control rather than for modulating the enzymatic functions of NS3-4A.

Source: Journal of Virology

DOI: 10.1128/jvi.00745-14

Authors: Andrew Kohlway, Nathan Pirakitikulr, Steve C. Ding, Feng Yang, Dahai Luo, Brett D. Lindenbach, Anna M. Pyle

Institutions: Yale University, Howard Hughes Medical Institute

Language: English

Topic: Hepatitis C virus research

Subfield: Hepatology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute of General Medical Sciences, National Institute of Allergy and Infectious Diseases, National Center for Advancing Translational Sciences

Awards: Predoctoral Program in Cellular and Molecular Biology, Medical Scientist Training Program, Yale University Clinical and Translational Science Award Program, Structure and function of the HCV replication complex

OpenAlex ID: W2169701242

FWCI: 1.913

Cites: 28

Cited by: 22

Related to: 10