Utilising inkjet printed paraffin wax for cell patterning applications
Year: 2015
Type: article
Abstract: We describe a method to prepare patterned environments for eukaryotic cells by inkjet printing paraffin wax onto a substrate. This technique bypasses the requirement to create a master mould, typically required with the use of polydimethylsiloxane techniques and the printed structure could be immediately used to guide cell proliferation. In a space of 2–3 hours, the desired pattern could be created with computer assisted design, printed and have cells seeded onto the scaffold, which could reduce the cycle time of prototyping micropattern designs. Human dermal fibroblasts and RN22 Schwann cells were seen to proliferate within the fabricated patterns and survive for more than 7 days. Additionally, the wax constructs could be readily removed from the substrate at any stage after cell seeding with the cells continuing to proliferate. Thus, we report on a simple but novel approach for the controlled physical positioning of live cells by wax inkjet printing.
Authors: Christopher Chi Wai Tse, Shea Shin Ng, Jonathan Stringer, Sheila MacNeil, John W. Haycock, Patrick J. Smith
Institution: University of Sheffield
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W2170515432