Characterization of incubation experiments and development of an enrichment culture capable of ammonium oxidation under iron-reducing conditions
Year: 2015
Type: article
Abstract: . Incubation experiments were conducted using soil samples from a forested riparian wetland where we have previously observed anaerobic ammonium oxidation coupled to iron reduction. Production of both nitrite and ferrous iron were measured repeatedly during incubations when the soil slurry was supplied with either ferrihydrite or goethite and ammonium chloride. Significant changes in the microbial community were observed after 180 days of incubation as well as in a continuous flow membrane reactor, using 16S rRNA gene PCR-denaturing gradient gel electrophoresis, 454-pyrosequencing, and real-time quantitative PCR analysis. We believe that one of the dominant microbial species in our system (an uncultured Acidimicrobiaceae bacterium A6), belonging to the Acidimicrobiaceae family, whose closest cultivated relative is Ferrimicrobium acidiphilum (with 92% identity) and Acidimicrobium ferrooxidans (with 90% identity), might play a key role in this anaerobic biological process that uses ferric iron as an electron acceptor while oxidizing ammonium to nitrite. After ammonium was oxidized to nitrite, nitrogen loss proceeded via denitrification and/or anammox.
Source: Biogeosciences
Authors: S. Huang, P. R. Jaffé
Institution: Princeton University
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $1,628
Funder: Princeton University
OpenAlex ID: W2170932381