Work

Mitochondrial Cristae Revealed with Focused Light

Year: 2009

Type: article

Abstract: Because of the diffraction resolution barrier, optical microscopes have so far failed in visualizing the mitochondrial cristae, that is, the folds of the inner membrane of this 200 to 400 nm diameter sized tubular organelle. Realizing a approximately 30 nm isotropic subdiffraction resolution in isoSTED fluorescence nanoscopy, we have visualized these essential structures in the mitochondria of intact cells. We find a pronounced heterogeneity in the cristae arrangements even within individual mitochondrial tubules.

Source: Nano Letters

DOI: 10.1021/nl901398t

Authors: Roman Schmidt, Christian A. Wurm, Annedore Punge, Alexander Egner, Stefan Jakobs, Stefan W. Hell

Institution: Max Planck Institute for Biophysical Chemistry

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2171000233

FWCI: 8.792

Cites: 14

Cited by: 159

Related to: 10