Isolation of the Oleaginous Yeasts from the Soil and Studies of Their Lipid-Producing Capacities
Year: 2009
Type: article
Abstract: D-xylose is one of the most abundant pentose sugars in nature. To isolate oleaginous yeasts that can utilize xylose from diverse soil samples, soils from a litchi orchard, longan orchard, carambola orchard, and woods were collected, yeasts were isolated by the glycerol enrichment and their xylose-assimilating capacities were measured. A subset of these isolates was grown in nitrogen-limited media and then screened for potential oleaginous yeasts by the Sudan Black B staining, after which their lipid-producing capacities were studied. There were 13 strains of oleaginous yeasts identified, and a rapid microbiological assay was provided to exploit microbial lipids that may one day be used as biodiesels or cocoa butter substitute.
Authors: Li-Xia Pan, Deng-Feng Yang, Li Shao, Wei Li, Gui-Guang Chen, Zhi-Qun Liang
Institutions: Guangxi University, Guangxi Academy of Sciences
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2171066132
