Normal Development, Wound Healing, and Adenovirus Susceptibility in β5-Deficient Mice
Year: 2000
Type: article
Abstract: Integrins have been shown to play important roles in embryonic development, wound healing, metastasis, and other biological processes. alphavbeta5 is a receptor for RGD-containing extracellular matrix proteins that has been suggested to be important in cutaneous wound healing and adenovirus infection. To examine the in vivo function of this receptor, we have generated mice lacking beta5 expression, using homologous recombination in embryonic stem cells. Mice homozygous for a null mutation of the beta5 subunit gene develop, grow, and reproduce normally. Keratinocytes harvested from beta5(-/-) mice demonstrate impaired migration on and adhesion to the alphavbeta5 ligand, vitronectin. However, the rate of healing of cutaneous wounds is not different in beta5(-/-) and beta5(+/+) mice. Furthermore, keratinocytes and airway epithelial cells obtained from null mice show adenovirus infection efficiency equal to that from wild-type mice. These data suggest that alphavbeta5 is not essential for normal development, reproduction, adenovirus infection, or the healing of cutaneous wounds.
Source: Molecular and Cellular Biology
Institutions: University of California, San Francisco, Gladstone Institutes
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G1106185146, HIV-1 REVERSE TRANSCRIPTION IN QUIESCENT LYMPHOCYTES, In Vivo Function of Pulmonary Integrins, Interactions of Lymphocytes, Cytokines and Airway Cells, G358049942, G722175103, In Vivo Function of Pulmonary Integrins, FUNCTION OF A NOVEL AIRWAY EPITHELIAL ADHESION RECEPTOR, G9000014683, G9000283680
OpenAlex ID: W2171118123