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Expression, Renaturation and Functional Analysis of an Excitatory Insect-Specific Toxin from Scorpion Buthus martensii Karsch

Year: 2005

Type: article

Abstract: The cDNA of BmK IT-AP, an excitatory insect toxin from the scorpion Buthus martensi Karsch that has an analgesic effect on mammalian cells, was expressed in E. coli in the form of an inclusion body. Following denaturation and reduction, the recombinant protein was renatured and purified by liquid chromatography. The authenticity of the recombinant product was confirmed by bioassay and its electrophysiological effect on insect sodium channel.

Source: Protein and Peptide Letters

DOI: 10.2174/0929866054696172

Authors: Chong Li, Wei Liu, Frank Bossmans, Rong-Huan Zhu, Jan Tytget, Da-Cheng Wang

Institutions: Chinese Academy of Sciences, Institute of Biophysics

Language: English

Topic: Venomous Animal Envenomation and Studies

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Natural Science Foundation of China

OpenAlex ID: W2171287382

FWCI: 0.539

Cites:

Cited by: 9

Related to: 10