Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes
Year: 2014
Type: article
Abstract: With defined culture protocol, human embryonic stem cells (hESCs) are able to generate cardiomyocytes in vitro, therefore providing a great model for human heart development, and holding great potential for cardiac disease therapies. In this study, we successfully generated a highly pure population of human cardiomyocytes (hCMs) (>95% cTnT(+)) from hESC line, which enabled us to identify and characterize an hCM-specific signature, at both the gene expression and DNA methylation levels. Gene functional association network and gene-disease network analyses of these hCM-enriched genes provide new insights into the mechanisms of hCM transcriptional regulation, and stand as an informative and rich resource for investigating cardiac gene functions and disease mechanisms. Moreover, we show that cardiac-structural genes and cardiac-transcription factors have distinct epigenetic mechanisms to regulate their gene expression, providing a better understanding of how the epigenetic machinery coordinates to regulate gene expression in different cell types.
Source: Protein & Cell
Institutions: Salk Institute for Biological Studies, Chinese Academy of Sciences, Institute of Biophysics, Shanghai Institute of Materia Medica, State Key Laboratory of Drug Research, University of California San Diego, Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, Center of Regenerative Medicine in Barcelona, University of Luxembourg, Luxembourg Centre for Systems Biomedicine
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,700
Funders: Ellison Medical Foundation, California Institute for Regenerative Medicine, Leona M. and Harry B. Helmsley Charitable Trust, Chinese Academy of Sciences, Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina, National Institutes of Health, Instituto de Salud Carlos III, State Key Laboratory of Drug Research, National Key Research and Development Program of China, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of General Medical Sciences, National Laboratory of Biomacromolecules
Awards: G1118969332, G326723367, Functional characterization of mutational load in nuclear reprogramming and differentiation, G5226670311, G579332670, G5952284444, G6391271827, REGULATORY GENOMIC STUDIES IN A COHORT OF IPS CELL DERIVED CARDIOMYOCYTES, Genome-methylome Interactions, Cancer Center Support Grant (CCSG)
OpenAlex ID: W2171351049