Work

Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps

Year: 2010

Type: article

Abstract: Advances in sequencing technology allow genomes to be sequenced at vastly decreased costs. However, the assembled data frequently are highly fragmented with many gaps. We present a practical approach that uses Illumina sequences to improve draft genome assemblies by aligning sequences against contig ends and performing local assemblies to produce gap-spanning contigs. The continuity of a draft genome can thus be substantially improved, often without the need to generate new data.

Source: Genome biology

DOI: 10.1186/gb-2010-11-4-r41

Authors: Isheng J Tsai, Thomas D Otto, Matthew Berriman

Institution: Wellcome Sanger Institute

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $5,490

Funder: Wellcome Trust