Work

Functional Expression of hMYH, a Human Homolog of the Escherichia coli MutY Protein

Year: 1999

Type: article

Abstract: We have previously described the hMYH cDNA and genomic clones (M. M. Slupska et al., J. Bacteriol. 178:3885-3892, 1996). Here, we report that the enzyme expressed from an hMYH cDNA clone in Escherichia coli complements the mutator phenotype in a mutY mutant and can remove A from an A. 8-hydroxydeoxyguanine mismatch and to a lesser extent can remove A from an A. G mismatch in vitro.

Source: Journal of Bacteriology

DOI: 10.1128/jb.181.19.6210-6213.1999

Authors: Malgorzata M. Slupska, Wendy M. Luther, Ju-Huei Chiang, Hanjing Yang, Jeffrey H. Miller

Institution: University of California, Los Angeles

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: SPONTANEOUS AND CARCINOGEN INDUCED MUTAGENESIS, G3341020898