Compartmentalized signaling of Ras in fission yeast
Year: 2006
Type: article
Abstract: Compartment-specific Ras signaling is an emerging paradigm that may explain the multiplex outputs from a single GTPase. The fission yeast, Schizosaccharomyces pombe, affords a simple system in which to study Ras signaling because it has a single Ras protein, Ras1, that regulates two distinct pathways: one that controls mating through a Byr2-mitogen-activated protein kinase cascade and one that signals through Scd1-Cdc42 to maintain elongated cell morphology. We generated Ras1 mutants that are restricted to either the endomembrane or the plasma membrane. Protein binding studies showed that each could interact with the effectors of both pathways. However, when examined in ras1 null cells, endomembrane-restricted Ras1 supported morphology but not mating, and, conversely, plasma membrane-restricted Ras1 supported mating but did not signal to Scd1-Cdc42. These observations provide a striking demonstration of compartment-specific Ras signaling and indicate that spatial specificity in the Ras pathway is evolutionarily conserved.
Institutions: Baylor College of Medicine, New York University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: G1867246295, Compartment Specific Signaling of Ras, Regulation of K-Ras by a Farnesyl-electrostatic Switch, A MULTI-SCALE APPROACH TO CELL STRUCTURE & FUNCTION, G3896083991, G4317585257, Structure/Function Analysis of Icmt, G6923034717, Selective control of multiple Ras signaling activities, G6965995057, REGULATION OF GENETIC STABILITY BY THE INT6 FAMILY, G9000050739, G905221223
OpenAlex ID: W2172047190