Effect of Abiotic Elicitors on Tissue Culture of ALOE vera
Year: 2014
Type: article
Abstract: Aloe vera L. belong to the Aloaceae family and actually is a commercial and medicinal important plant because of the secondary metabolites. Anthraquinones are well- known and widely used in medicine and food chemistry. It has dermatological usage with antimalarial, antifungal and antibacterial properties. Aloin is the most important secondary metabolite in Aloe vera, not only due to the medicinal and cosmetic properties but also due to their physiological role. Plant cell cultures have been used industrially for the synthesis of secondary metabolites. In this study we have investigated the effects of different abiotic elicitors including Nano-Ag, NanoTio2, NH4NO3 and sucrose on cell suspension culture of Aloe vera. The induced callus by elicitors were collected in five period of time and have been analyzed by HPLC (High-performance liquid chromatography). The results showed that the highest amount of aloin obtained with NH4NO3 at 48hours after treatment, and it was 127% higher than control. Aloin content was enhanced with Nano elicitors at 48 hours after treatment and decreased gradually after that. Moreover, aloin was increased gradually up to168 hours after sucrose treatment. These experiments showed that in vitro culture of Aloe vera can be induced to produce more secondary metabolites.
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2174470053