Work

In Situ and In Vivo Molecular Analysis by Coherent Raman Scattering Microscopy

Year: 2016

Type: review

Abstract: Coherent Raman scattering (CRS) microscopy is a high-speed vibrational imaging platform with the ability to visualize the chemical content of a living specimen by using molecular vibrational fingerprints. We review technical advances and biological applications of CRS microscopy. The basic theory of CRS and the state-of-the-art instrumentation of a CRS microscope are presented. We further summarize and compare the algorithms that are used to separate the Raman signal from the nonresonant background, to denoise a CRS image, and to decompose a hyperspectral CRS image into concentration maps of principal components. Important applications of single-frequency and hyperspectral CRS microscopy are highlighted. Potential directions of CRS microscopy are discussed.

Source: Annual Review of Analytical Chemistry

DOI: 10.1146/annurev-anchem-071015-041627

Authors: Chien-Sheng Liao, Ji-Xin Cheng

Institution: Purdue University West Lafayette

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green