Work

Integrity of the Exon 6 Sequence Is Essential for Tissue-Specific Alternative Splicing of Human Leukocyte Common Antigen Pre-mRNA

Year: 1989

Type: article

Abstract: By alternative splicing, exons 4, 5, and 6 of the human leukocyte common antigen (LCA) gene are included in B-cell mRNA but excluded from thymocyte mRNA. A mini-LCA gene that contains only LCA exons 2, 6, and 8 faithfully reproduces this tissue-specific alternative splicing in mouse B and thymocyte cell lines. Elimination of almost all of the intron sequences associated with exon 6 had no effect on the alternative splicing, while linker-scanning analysis showed that a significant length of the exon 6 sequence is essential for alternative splicing.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.9.10.4550

Authors: A Y Tsai, M Streuli, H Saito

Institutions: Harvard University, Dana-Farber Cancer Institute

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of Allergy and Infectious Diseases, National Institute of Dental and Craniofacial Research

Awards: G6273719209, G7960804125