Year: 2001
Type: dissertation
Abstract: Estimates of variance parameters, such as heritability, describing quantitative genetic traits in natural populations are of scientific interest in a number of fields such as conservation and evolutionary biology.Central to the estimation of heritability is the covariance of the trait between individuals of known relationship.In natural populations, however, exact relationships may be unknown.In situations where molecular marker data are available, information can be inferred about the relationships without reference to an exact pedigree, and this information may be used to estimate the heritability.Two existing estimators use inferred relationship information on a pair-wise level: regression of the phenotypic similarity of a pair of individuals on an estimate of their relationship and a likelihood procedure that maximises the probability of their genotypic and phenotypic observations.Computer simulation was used to compare the behaviour of these approaches.Bias in estimates of heritability decreased with increasing sample size, decreased simulated heritability, increasing relatedness and increasing sample size.The regression approach showed less bias than the likelihood approach, but much larger sampling variance.A modified form of the likelihood technique, requiring fewer initial assumptions about population parameters was developed, which showed lower bias in its estimates of heritability than the likelihood technique originally proposed.An alternative approach in which marker-information was used to reconstruct sibships through relationship assignment within a single generation using Markov chain Monte Carlo (MCMC) techniques was developed.The reconstructed sibships were assumed correct and analysed using restricted maximum likelihood under an animal model.Simulations to compare the properties of estimates with those made using existing techniques indicated that sibship reconstruction was, in many cases, superior to earlier methods, regaining family-specific weighting lost through pairwise analysis, having lower mean squared errors and showing only slight downwards bias, provided that there was sufficient marker information.Equations appropriate for MCMC analysis of half-sib, full-sib and hierarchical sib-ship structures are presented.The approaches were extended so that information from other types of marker loci, for example mitochondrial or dominant loci, known maternal information and additional variance parameters can be incorporated into the analysis.Analysis using the technique was made of a feral population of Soay sheep, with body weight being used as an example trait.Results indicated that the Soay population has a low level of relatedness and so heritability estimates were not reliable, unless inferred relationship data was used only to augment an existing set of known relationships.In conclusion, the described methods show considerable promise, but are restricted by the need for large family sizes or, equivalently, a large variance of relationship in the sample.In addition they require that about ten polymorphic marker loci be typed per individual before estimated heritabilities become reliable, unless known relationships are also included in the analysis.In consequence they will not be appropriate for all natural populations of interest.I would like to thank the following for their help in the preparation of this thesis.-My supervisors, Professor Bill Hill and Doctor Josephine Pemberton for their comments, discussions and advice.In particular, I would like to thank Bill for his patience during impromptu statistics lessons and Josephine for her handling of matters bureaucratic.-Professor Nick Barton for suggestions regarding Chapter 4; his words, if memory serves, "If we move someone from here to here [hand gestures are left as an exercise for the reader] and see how the likelihood of the parameters changes."-My officemates for screening my work (both scientific and random), before it faced the scrutiny of my supervisors.-My family and Katherine, for their support and encouragement over the past-three years, and their distractions when distractions were required.
Source: ERA
Author: Stuart C. Thomas
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2177331017