Work

Efficient plant regeneration of Malaysian aromatic rice (Oryza sativa L.) via improved somatic embryogenesis pathway

Year: 2015

Type: article

Abstract: Aromatic rice variety namely MRQ 74, MRQ 80 and MRQ 50 are the most popular rice in Malaysia.Establishing an efficient regeneration protocols via somatic embryogenesis is required for varietal improvements.An efficient somatic embryogenesis system has been established for three aromatic rice varieties using mature seeds.MS-B5 medium containing 1 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 10 mg/L NAA (α-naphthaleneacetic acid) optimized that produced highest embryogenic callus (89%) without any browning effect.The highest whitish somatic embryos frequency (75%) was initiated by incubating embryogenic calli on same medium containing 10 mg/L ABA and 9 g/L Gelrite for 8 weeks.However, the numbers of regenerated plantlets on medium containing NAA and 2,4-D which was previously pre-treated with 10 mg/L ABA, 9 g/L Gelrite and incubation at 8 weeks was the best method for shoots induction of three rice varieties.

Source: Emirates Journal of Food and Agriculture

DOI: 10.9755/ejfa.2015-07-535