Year: 2013
Type: conference-paper
Abstract: Förster resonance energy transfer (FRET) provides fluorescence signals sensitive to intra- and inter-molecular distances in the 1-10 nm range. Widely applied in the optical imaging environment, FRET enables visualization of physicochemical processes in molecular interactions and conformation changes. We reported photoacoustic imaging of FRET, based on non-radiative decay that produces heat and subsequent acoustic waves. The experimental results show that photoacoustic imaging offers better penetration into scattering biological tissue. Through its ability to three-dimensionally image tissue with scalable resolution, photoacoustic microscopy provides a beneficial biomedical tool to broaden the in vivo application of the FRET technique.
DOI: 10.1117/12.2004150
Authors: Yu Wang, Lihong V. Wang
Institution: Washington University in St. Louis
Language: Swedish
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2178128997