Work

Minimally invasive microendoscopy system for in vivo functional imaging of deep nuclei in the mouse brain

Year: 2015

Type: article

Abstract: The ability to image neurons anywhere in the mammalian brain is a major goal of optical microscopy. Here we describe a minimally invasive microendoscopy system for studying the morphology and function of neurons at depth. Utilizing a guide cannula with an ultrathin wall, we demonstrated in vivo two-photon fluorescence imaging of deeply buried nuclei such as the striatum (2.5 mm depth), substantia nigra (4.4 mm depth) and lateral hypothalamus (5.0 mm depth) in mouse brain. We reported, for the first time, the observation of neuronal activity with subcellular resolution in the lateral hypothalamus and substantia nigra of head-fixed awake mice.

Source: Biomedical Optics Express

DOI: 10.1364/boe.6.004546

Authors: Miriam E. Bocarsly, Wan-chen Jiang, Chen Wang, Joshua T. Dudman, Na Ji, Yeka Aponte

Institutions: National Institute on Drug Abuse, Howard Hughes Medical Institute, Janelia Research Campus, National Institute on Alcohol Abuse and Alcoholism

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,913

Funders: Howard Hughes Medical Institute, National Institute on Drug Abuse