Work

Aligning the μs-ALEX Setup

Year: 2015

Type: preprint

Abstract: To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G(550-620)R(650-750).

Source: Cold Spring Harbor Protocols

DOI: 10.1101/pdb.prot086421

Authors: Achillefs Kapanidis, Devdoot Majumdar, Mike Heilemann, Eyal Nir, Shimon Weiss

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2180779742

Cites: 3

Cited by: 4

Related to: 10