Integrative Study on PPARGC1A: Hypothalamic Expression of Ppargc1a in ob/ob Mice and Association between PPARGC1A and Obesity in Korean Population
Year: 2008
Type: article
Study design: Observational Study
Abstract: Obesity is an increasing worldwide health problem\nthat is strongly related to the imbalance of food intake\nand energy metabolism. It was well-known that several\nsubstances in the hypothalamus regulate food\nintake and energy metabolism. We planned an integrative\nstudy to elucidate the mechanism of the development\nof obesity. Firstly, to find candidate genes\nwith the marvelous effect, the different expression in\nthe hypothalamus between ob/ob and 48-h fasting\nmice was investigated by using DNA microarray technology.\nAs a result, we found 3 genes [peroxisome\nproliferator activated receptor, gamma, coactivator\n1 alpha (Ppargc1a), calmodulin 1 (Calm1), and complexin\n2 (Cplx2)] showing the different hypothalamic\nexpression between ob/ob and 48-h fasting mice.\nSecondly, a genetic approach on PPARGC1A gene\nwas performed, because PPARGC1A acts as a transcriptional\ncoactivator and a metabolic regulator.\nTwo hundred forty three obese female patients with\nbody mass index (BMI)›25 and 285 control female\nsubjects with BMI 18 to ⁄23 were recruited according\nto the Classification of Korean Society for the Study\nof Obesity. Among the coding single nucleotide\npolymorphisms (cSNPs) of PPARGC1A, 2 missense\nSNPs (rs8192678, Gly482Ser; rs3736265, Thr612Met)\nand 1 synonymous SNP (rs3755863, Thr528Thr) were\nselected, and analyzed by PCR-RFLP and pyrosequencing.\nFor the analysis of genetic data, chi-square\n(X2) test and EH program were used. The rs8192678\nwas significantly associated with obese women (P⁄\n0.0006; odds ratio, 1.5327; 95% confidence interval,\n1.2006-1.9568). Haplotypes also showed significant\nassociation with obese women (X2=33.28, P⁄0.0008).\nThese results suggest that PPARGC1A might be related\nto the development of obesity.\nKeywords: Hypothalamus, Microarray, ob/ob Mice,\nObesity, Polymorphism, PPARGC1A
Language: English
Subfield: Endocrine and Autonomic Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2182821084