Anti-idiotypic regulation of cofactor- -independent antiphospholipid antibodies
Year: 2011
Type: article
Abstract: Background: Antiphospholipidantibodies(APA)responsedevelopmentandregulationisstillunclear, thereforeAPAclinicalroleorassociationisstillondebate.InourpreviousstudythedifferenceinAPA specificityandcofactor-dependencyinpatientswithautoimmuneandnonautoimmunediseaseswas shown.InthisinvestigationwestudiedAPA-antiidiotypicantibodies(Abs)networkinchronicviral hepatitisB,systemiclupuserythematosus(SLE)patientsandinwomenundergoinginvitrofertilization (IVF). Material and methods:Seraof32patientswithSLE,30withchronicviralhepatitisB,142women undergoingIVFand82healthyindividualswereinspectedforAPAandanti-idiotypicantiphospholipid antibodies(AiAPA).Anti-idiotypicantiphospholipidantibodieswereexaminedbytwodifferentmethods: 1) direct binding AiAPA in ELISA with mouse monoclonal cofactor-independent APA (mAPA); 2) antiidiotypic neutralization of monoclonal APA reaction with phospholipids; antiphospholipid antibodiespositivewomenundergoingIVFweretreatedbylowdoseintravenousimmunoglobulin(IvIg), theirserumwastestedforAPAandAiAPAlevelsbeforeand5dayaftertreatment. Results:InAPA+womenundergoingIVFthemeanlevelofAiAPAwassignificantlowerthanin APA-womeninthesamegroup(p<0.05).Intravenousimmunoglobulin(IvIg)infusionauthentically reducedantiphospholipidantibodiesaswellasraisedAiAPAlevelsinAPA+womenundergoingIVF. Conclusion: Result of this research confirmed a natural AiAPA participation in regulation of productioncofactor-independentAPA.
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W2183310209