Work

A Standardized Kit for Automated Quantitative Assessment of Candidate Protein Biomarkers in Human Plasma

Year: 2015

Type: article

Abstract: BACKGROUND: An increasingly popular mass spectrometry-based quantitative approach for health-related research in the biomedical field involves the use of stable isotope-labeled standards (SIS) and multiple/selected reaction monitoring (MRM/SRM). To improve inter-laboratory precision and enable more widespread use of this 'absolute' quantitative technique in disease-biomarker assessment studies, methods must be standardized. Results/methodology: Using this MRM-with-SIS-peptide approach, we developed an automated method (encompassing sample preparation, processing and analysis) for quantifying 76 candidate protein markers (spanning >4 orders of magnitude in concentration) in neat human plasma. DISCUSSION/CONCLUSION: The assembled biomarker assessment kit - the 'BAK-76' - contains the essential materials (SIS mixes), methods (for acquisition and analysis), and tools (Qualis-SIS software) for performing biomarker discovery or verification studies in a rapid and standardized manner.

Source: Bioanalysis

DOI: 10.4155/bio.15.222

Authors: Andrew J Percy, Yassene Mohammed, Juncong Yang, Christoph H Borchers

Institutions: University of Victoria, Genome British Columbia, Leiden University Medical Center

Language: English

Topic: Advanced Proteomics Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Funder: Hospital for Sick Children