Year: 2011
Type: article
Abstract: Controlled ozone administration has been shown to promote an adaptation to oxidativestress by increasing endogenous antioxidant systems. In the present study, the effectsof O2/O3 administration either prophylactically or therapeutically on the alterations ofoxidant status in adjuvant-induced arthritis in rats have been studied. Seven groupsof rats were used: 1) normal control group; 2) control arthritic group (21 days); 3) prophylacticozone group: arthritic rats received fifteen intra-rectal applications of O2/O3at 0.5, 0.7 and 1 mg/kg b.w. in a 5-6 mL volume starting one day before adjuvant inoculationand continued as five applications/week over 21 days; 4) oxygen group: receivedoxygen (vehicle of ozone) in a similar schedule to group 3; 5) control arthriticgroup (24 days); 6) therapeutic-ozone group: arthritic rats received 10 intra-rectal applicationsof O2/O3 at 0.5, 0.7 and 1 mg/kg b. w. in a 5-6 mL volume daily for 10 daysstarting fourteen days after adjuva nt i noculation; 7) oxygen-trea ted group: receivedoxygen in a similar schedule of group 6. The effect of O2/O3 administration was assessedby measuring: bloo d glutathione (GSH ), erythrocyte glutathione peroxidaseand catalase activities, serum levels of protein thiols (PrSH), malondialdehyde (MDA)and nitrite/nitrate (NO2/NO3), as well as serum ceruloplasmin activity (CP). The presentstudy showed that adjuvant-induced arthritis in rats caused a significant (p<0.05)reduction in blood GSH, serum PrSH levels and erythrocyte antioxidant enzyme activitiesa ccompanied b y a significant (p<0 .05) i ncreas e in s erum le vels of MDA,NO2/NO3 and CP activity. Ozone administration either prophylactically or therapeuticallynormalize blood GSH, serum PrSH and MDA levels and restored erythrocyte antioxidantenzyme activities. However ozone did not significantly (p>0.05) modify serumNO2/NO3 level in induced rat but significant (p<0.05) increase CP activity. So it couldbe concluded that O2/O3 oxidative preconditioning / postconditioning effectively modulatethe antioxidant/oxidant balance associated with adjuvant arthritis model in rats.
Source: Ozone Therapy Global Journal
DOI: 10.67442/3aj5zh78
Language: English
Subfield: Pharmacology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W1849519521