DNA-Based MRI Probes for Specific Detection of Chronic Exposure to Amphetamine in Living Brains
Year: 2009
Type: article
Abstract: We designed phosphorothioate-modified DNA probes linked to superparamagnetic iron oxide nanoparticles (SPION) for in vivo magnetic resonance imaging (MRI) of fosB and Delta fosB mRNA after amphetamine (AMPH) exposure in mice. Specificity of both the fosB and Delta fosB probes was verified by in vitro reverse transcriptase-PCR amplification to a single fragment of total cDNA obtained from acutely AMPH-exposed mouse brains. We confirmed time-dependent uptake and retention profiles of both probes in neurons of GAD67-green fluorescent protein knock-in mice. MRI signal of SPION-labeled fosB probe delivered via intracerebroventricular route was elevated in both acutely and chronically AMPH-exposed mice; the signal was suppressed by dopaminergic receptor antagonist pretreatment. SPION-labeled Delta fosB probe signal elevation occurred only in chronically AMPH-exposed mice. The in vivo target specificity of these probes permits reliable MRI visualization of AMPH-induced differential elevations of fosB and Delta fosB mRNA in living brains.
Source: Journal of Neuroscience
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Awards: In vivo Profiling of Glial and Neuronal Activities in Psychostimulant Abuse, Neurovascular Mechanisns of Brain Function and Disease, Center for Functional Imaging Technologies, Cocaine and Brain Development, Interdepartmental Neuroscience Center, Perfusion Imaging with Magnetic Resonance, Automated Alignment of MRI Rodent Brain to Sterotaxic Atlases, Gene Repair in signal Transduction after CNS Injury, MR Assessment of Altered Cerebral Gene Expression after Amphetamine Exposure
OpenAlex ID: W1985285230