Year: 2013
Type: article
Abstract: Ruscus hypoglossum L. is a highly valued ornamental plant collected mainly from the wild and threatened in several Europeancountries. Slow growth and low germination rates hamper its cultivation. Micropropagation has been considered advantageousfor rapid production of planting material and ex situ conservation of Ruscus species. Callus cultures of R. hypoglossumwere induced on TDZ containing medium and indirect shoot regeneration rate was evaluated on different medium composition.TDZ ensured higher regeneration rates, caused shoot, and cladode alterations in regenerates persisting throughout thecultivation. Callus induction was optimal at 30 g.l–1 sucrose and dropped down with the increase of sucrose concentration. Increaseof the sucrose (15–60 g.l–1) infl uenced positively shoot proliferation. DNA content of the morphologically altered plantswas not significantly different. Isozyme profile patterns demonstrated loss of isoform bands and/or induction of new ones.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2184358344