Agreement Between Estimated Apolipoprotein B -100 Concentration and Apolipoprotein B-100 Concentration Measured by Immunoturbidimetric Method
Year: 2014
Type: article
Study design: Observational Study
Abstract: Apo B-100 is synthesized in the liver and only one apo B molecule exists in every LDL, IDL and VLDL particle, therefore, the level ofapo B-100 represents the total number of potentially atherogenic lipid. A formula estimating apo B-100 might help clinicians inareas with limited resources to identify patients with risk factors for cardiovascular disease, so proper management can be carriedout. To evaluate an agreement between estimated apolipoprotein B-100 concentration and apolipoprotein B-100 concentrationmeasured by immunoturbidimetric method. This study was an analytical observational cross sectional design. Serum samples werecollected using simple random sampling method and all specimens were measured for LDL-cholesterol, triglyceride andapolipoproten B-100 concentration. Estimated apo B-100 concentrations were counted using a [Apo B-100= -33.12 + 0.675 x LDL+ 11.95x ln (TG)] formula. Twenty nine of 30 samples (96.67%) showed estimated apo B-100 concentrations higher than measuredapo B-100 concentrations. Difference of the mean of estimated and measured apo B-100 concentration was 10.941 mg/dL, and wasstatistically significant (t= 9.134 (p <0.0001)). Concordance correlation coefficient (CCC) between estimated and measuredapolipoprotein B-100 concentration was 0.854 (<0.90= poor agreement). The agreement between estimated apo B-100concentration and apo B-100 concentration measured with immunoturbidimetric method was poor. Factors that might have causedthis result were freeze-thawing of samples prior to apo B-100 measurement using immunoturbidimetry and the use of triglyceride asone of the variables in the formula in estimating apo B-100.(FMI 2014;50 10-14)
Source: Folia Medica Indonesiana
Language: English
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $98
OpenAlex ID: W2184575230