Work

Pulse Chase of Suspension Cells

Year: 2014

Type: article

Abstract: Pulse-chase method is a powerful technique used to follow the dynamics of proteins over a period of time. The expression level, processing, transport, secretion or half-life of proteins can be tracked by metabolically labeling the cells, such as with radiolabeled amino acids (pulse step). This protocol describes the condition used to study the folding and disulfide bond formation of immunoglobulin in suspension cells. With some minor modifications, this protocol can be adapted to study the degradation rate or the secretion of target proteins.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.1170

Authors: Lai-Yee Wong, QiMing Liang, Kevin Brulois, Jae Jung

Institution: University of Southern California

Language: English

Topic: Viral Infectious Diseases and Gene Expression in Insects

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Cancer Institute, National Institute of Allergy and Infectious Diseases

Awards: G1850526206, G3963602007, G4293093837, G5641079710, G6918348011