A simple and rapid scanning electron microscope preparative technique for observation of biological samples: application on bacteria and DNA samples
Year: 2013
Type: article
Abstract: This protocol details a rapid preparative procedure enabling the visualization of biological samples like DNA and bacteria by scanning electron microscopy (SEM). This involves the fixation of the samples, followed by subsequent dehydration and the processing for electron microscopy observation. The fixation step is performed by allowing the samples to embed into an agar matrix. The dehydration of the samples ensures that they will not disrupt when processing for SEM observation or in the SEM vacuum camera. The whole process may be achieved for 6–24 hours and has been optimized for the visualization of bacteria, DNA. Samples once processed for SEM can be stored mild vacuum for weeks, allowing sufficient time for image acquisition.
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2186024592