Work

Quantitative Analysis of RNA Modifications

Year: 2010

Type: book-chapter

Abstract: RNA modifications impact numerous cellular processes such as pre-mRNA splicing and protein synthesis. The elucidation of the mechanisms by which these modifications impact cellular processes necessitates the ability to both detect and quantify the presence of these modifications within RNA molecules. Here, we present a detailed procedure that allows the detection and quantification of RNA base modifications. This procedure involves a number of techniques, including oligonucleotide-affinity selection, site-specific cleavage and radiolabeling, nuclease digestion, and thin layer chromatography.

Source: Methods in molecular biology

DOI: 10.1007/978-1-60761-657-3_2

Authors: John Karijolich, Athena Kantartzis, Yi-Tao Yu

Institutions: University of Rochester Medicine, University of Rochester

Language: English

Topic: RNA modifications and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: G2100436727, Spliceosomal snRNA modification in Xenopus oocytes, G508708373, Training in Cellular, Biochemical and Molecular Sciences