Work

Negative Regulatory Effects of Phosphatidylinositol3-kinase Pathway on Phagocytosis and Macropinocytosis in Bovine Monocytes.

Year: 2014

Type: article

Abstract: transformed with a green fluorescence plasmid GFP to evaluate macropinocytosis, mannose receptor-mediated endocytosis, and phagocytosis in bovine professional antigen presenting cells, respectively. Our data reveal that wortmannin, an inhibitor of phosphatidylinositol 3-kinase signaling pathway, significantly increased macropinocytosis and phagocytosis but did not affect the mannose receptor-mediated antigen uptake in bovine monocytes. Protein expression data support these findings by showing decreased levels of phosphoinositide 3-kinase in the presence of wortmannin during macropinocytosis. We expanded further the key role of phosphatidylinositol 3-kinase as an endogenous suppressor of primary immune responses, suggesting a novel mechanism of phosphatidylinositol 3-kinase antigen uptake modulation that may provide a unique therapeutic target for controlling excessive inflammation.

Source: PubMed

Authors: Mais G Ammari, Autumn N Harris, John V Stokes, Richard H Bailey, Lesya M Pinchuk

Institutions: Mississippi State University, University of Arizona, University of Florida

Language: English

Topic: Cellular transport and secretion

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institutes of Health

Award: Summer Research Experience for Veterinary Students