Growth response and antioxidant enzyme activity of Cassia occidentalis exposed to soil moisture stress
Year: 2015
Type: article
Abstract: ABSTRACT Biotic and abiotic stresses exert a considerable influence on the growth and physiology of plants. Water stress is one of the most important abiotic stress factors. The study was carried out to elucidate the effect of drought stress on growth, physiology and antioxidant enzyme activity of Cassia occidentalis experiencing four water regimes with soil relative water content ranging from 65% to 12%. Plants were grown in pots and treated with three irrigation levels (100ml, 200ml and 300ml).The experiment was laid out in complete randomized design (CRD) with three replicates. Data on growth, physiological parameters and antioxidant enzyme activity were recorded and analyzed statistically. Physiological parameters like leaf area index, biomass accumulation, relative water content and membrane stability index decreased significantly with decrease in water level, but mild level of water treatment enhanced the growth. The plant exhibited an increase in antioxidant enzyme activity under stressed condition which proves its tolerance against drought. Thus it can be concluded that Cassia occidentalis plant is able to tolerate a limited water levels by enhancing its antioxidant defense but excessive drought markedly affected the enzyme activity and so depleted the plant growth. Key words : Relative water content, biomass, membrane stability index, leaf area index, antioxidant enzymes, stress. REFERENCES Apel, K. and H. Hirt 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol.55:373-399. Arora, A., R.K. Sairam, and G.C. Srivastava 2002. Oxidative stresses and antioxidative system in plants. Curr. Sci. 82: 1227-1237. Asada, K. (2006). Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol. 141: 391-396. Chance, B and A.C. Maehly, 1955. Assay of catalase and peroxidase. Methods in Enzymology 2:764-775. Cunhua, S., Wei, D., Xiangling, C.,Xinna, X., Yahong, X., Dong, S., and Jianjie, S. 2010. The effects of drought stress on the activity of acid phosphatase and its protective enzymes in pigweed leaves. African Journal of Biotechnology Vol. 9(6), pp. 825-833. Dalal, M. and Khanna-chopra R. 2001. Differential response of antioxidant enzymes in leaves of necrotic wheat hybrids and their parents. Physiol. Plant., 111:297-304. Deblonde, P.M.K., Haverkort A.J. and Ledent, J.F. 1999. Responses of early and late potato cultivars to moderate drought conditions. Agronomic parameters and carbon isotope discrimination. Eur J Agron 11(2):91-105 Deshmuukh, P.S., Sairam, R.K. and Shukla, D.S. 1991. Measurement of ion leakage as a screening technique for drought resistance in wheat genotypes. Indian journal of plant physiology, 34:89-91 Giannopolites, C.N. and S.K. Ries, 1977. Superoxide Dismutase I. Occurrence in higher plants. Plant Physiology. 59:309-314. Hennebelle, T., Weniger, B., Joseph, H., Sahpaz, S., Bailleul, F. Senna alata. Fitoterapia, 80: 385-393 (2009). Jefferies, R.A., and MacKerron, D.K.L. 1987. Aspects of physiological basis of cultivar differences in yield of potato under droughted and irrigated conditions. Potato Res 30:201-217. Kaya, C., Kirnak, H.D., Higgs, and Saltali., K. 2002. Supplementary calcium enhances plant growth and fruit yield in strawberry cultivars grown at high (NaCl) salinity. Sci. Hort., 93: 65-74. Khammari, I., Galavi, M., Ghanbari, A., Solouki, M., and Mohammad Reza Asghari Poorchaman. 2012. The effect of drought stress and nitrogen levels on antioxidant enzymes, proline and yield of Indian Senna (Cassia angustifolia L.). Journal of Medicinal Plants Research Vol. 6(11), pp. 2125-2130. Lahlou, O. and Ledent, J.F. 2005. Root mass and depth, stolons and roots formed on stolons in four cultivers of potato under stress. Eur J Argon 22:159-173. Moussa, H.R. and Moustafa. S 2008. Comparative response of drought tolerant and drought sensitive maize genotypes to water stress Australian Journal of Crop Science Southern Cross Journals 1(1):31-36. Parida, A.K., Dagaonkar, V.S., Phalak, M.S., Umalkar, G.V., Aurangabadkar 2007. Alterations in photosynthesis pigments, protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery. Plant Biotechnol. Rep., 1:37-48. Paritha bhanu and M. Deepak. The effect of Cadmium on antioxidant enzymes in the liver of fresh water fish Cyprius carpio (Linn). Biolife 2015;3(1); 50-53 Praba, M.L., Cairns, J.E., Babu, R.C., Lafitte, H.R. 2009. Identification of physiological traits underlying cultivar differences in drought tolerance in rice and wheat J Agron Crop Sci. 195: 30-46 Ramakrishna, N., Lacey, J. and Smith, J. E. 1991. Effect of surface sterilization, fumigation and gamma irradiation on the microflora and germination of barley seeds. Int. J. Food microbial., 13: 47-54. Sairam, R.K., Srivastava, G.C. and Saxena, D.C. 2000. Increased antioxidant activity under elevated temperature: a mechanism of heat stress tolerance in wheat genotypes. Biol. Plant. 43:245-251. Saraswati, S.G. and K. Paliwal 2008. Diurnal and seasonal treands in photosynthetic performance of Dalbergia sissoo Roxb. And Hardwickia binata Roxb. From a semi-arid ecosystem. Photosynthetica, 46, 248-254. Saraswati, S.G. and Paliwal K. 2011. Drought induced changes in growth, leaf gas exchange and biomass production in Albizia lebbeck and Cassia siamia seedlings. J. Environ. Biol. 32, 173- 178. Schonfeld, M.A., R.C. Johnson, B.F. Carver, and D.W. Mornhingweg 1988. Water relations in winter wheat as drought resistance indicator. Crop Sci. 28:526-531. Shannon, L.M., Kay, E. and Lew, J.Y. 1966. Peroxidase isoenzymes from horse radish roots I. Isolation and physiological properties. J. Biol. Chem. 241:2166-2172. Singh, B.P., 1970. The measurement of leaf area in dwarf wheat. Madrash Agri.J. 57: 296-298. Srivastava, S. Kumar, K. and Srivastava, M. 2012. Effect of different pre-sowing treatments on seed germination of Cassia occidentalis. Res. Environ.Life Sci. 5(3) 153-155. Staniszewska, I., A. Krolicka, E. Malinski, E. Lojkowska and J. Szafranek. 2003. Elicitation of secondary metabolites in in vitro cultures of Ammi majus L. Enzymes Microb. Technol., 33: 565-568. Xu. Z.-Z. and Zhou, G.-S. 2005. Effects of water stress and high nocturnal temperature on photosynthesis and nitrogen level of a perennial grass Leymus chinensis Plant and Soil 269: 131–139.
Source: Biolife
Author: Srishti Srivastava
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2188742447