Work

Use of Bromodeoxyuridine Immunohistochemistry for the Study of Cytogenesis and Neurochemical and Cellular Differentiation in the Marsupial Brain and Retina

Year: 1997

Type: article

Abstract: Determining the birthdate of neurons and glia in relation lO the time period of neurochemical 1.nd cellular differentiation are necessary for understanding early developmental events in the central nerv.>us system (CNS). Bromodeoxyuridine (BrdU). a thymidine analog has recently become an alternative to the tritiated thymidine autoradiography for the study of cytogenesis in the CNS. Our laboratory utilizes the Brazilian opossum, Monodelphis domestica to study the ontogeny of neuropeptide systems and the development of the visual system in the mammalian brain. Opossum pups are born in an extremely immature state before neurogenesis and morphogenesis are completed and provide an excellent experimental model for developmental studies. To this end, we have developed single, double. and triple label BrctU immunohistochemical procedures for simultaneous study of cytogenesis and cellular differentiation in the opossum brain and retina. These methods are simple, rapid, and very effective and could be widely applied for understanding developmental events in the CNS.

Source: Iowa State University Digital Repository (Iowa State University)

Authors: Javed Iqbal, Donald S. Sakaguchi, Chris R. Jensen, Carol D. Jacobson

Language: English

Topic: Neurogenesis and neuroplasticity mechanisms

Subfield: Developmental Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: green

Funders: National Science Foundation, Iowa State University

OpenAlex ID: W2188905693

FWCI:

Cites: 8

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Related to: 10