Role of transcriptional factor Nrf2 in the acute lung injury of mice.
Year: 2015
Type: article
Abstract: OBJECTIVE: This study aimed to investigate the expression and role of Nrf2 in the acute lung injury (ALI) of mice. METHODS: A total of 60 BABL/c mice were randomly divided into 2 groups: ALI group and control group. In ALI group, ALI was introduced by injection of LPS. Immunohistochemistry was performed to detect Nrf2 expression in the lung; Western blot assay was employed to detect the expression of Nrf2 in the lung homogenate; ELISA was conducted to detect the expression of Nrf2 in the lung homogenate and BALF. RESULTS: As compared to control group, ALI mice had a high Nrf2 expression in the lung as shown in immunohistochemistry, and the Nrf2 expression in the lung homogenate and BALF also increased markedly (P<0.05). CONCLUSION: The Nrf2 expression increases in the lung and BALF of ALI mice, suggesting that Nrf2 is involved in the inflammation during ALI and may serve as a new target in the therapy of ALI.
Source: PubMed
Authors: Minghao Kong, Jing Mao, Bijun Luo, Zonghao Qin
Institutions: Tongji University, Shanghai Tenth People's Hospital
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2194725203