Regulation of estrogen receptor signaling in breast and endometrial cancer by the src kinase pathway, the micronutrient selenium, and by novel tamoxifen-regulated biomarkers
Year: 2005
Type: article
Abstract: Tamoxifen is the primary hormonal therapy for breast cancer and is also used as a breast cancer chemopreventative agent.A major problem with tamoxifen therapy is undesirable endometrial proliferation.To identify proteins associated with the growth stimulatory effects of tamoxifen in an ER-positive model, the present study profiled total cellular and secreted proteins regulated by estradiol and selective estrogen receptor modifiers (SERMs) in the Ishikawa endometrial adenocarcinoma cell-line using twodimensional gel electrophoresis.Following 24-hour incubation with 10 -8 M estradiol, 10 -7 M 4-hydroxytamoxifen, or 10 -7 M EM-652 (Acolbifene), nine proteins exhibited significant increase in expression.The proteins identified were heat shock protein 90-α, and -β, heterogeneous nuclear ribonucleoprotein F, RNA polymerase II-mediating protein, cytoskeletal keratin 8, cytoskeletal keratin 18, ubiquitin-conjugating enzyme E2-18kDa and nucleoside diphosphate kinase B. These protein profiles may serve as novel indices of SERM response and may also provide insight into novel mechanisms of SERM-mediated growth.
Author: Yatrik M. Shah
Institution: University of Toledo
Language: English
Subfield: Endocrinology
Domain: Life Sciences
Open Access status: green
Funder: National Institutes of Health
OpenAlex ID: W2197953426