Work

Crystallographic Tool Box(CrysTBox): automated tools for transmission electron microscopists and crystallographers

Year: 2015

Type: article

Abstract: Three tools for an automated analysis of electron diffraction pattern and crystallographic visualization are presented. Firstly,diffractGUIdetermines the zone axis from selected area diffraction, convergent beam diffraction or nanodiffraction patterns and allows for indexing of individual reflections. Secondly,ringGUIidentifies crystallographic planes corresponding to the depicted rings in the ring diffraction pattern and can select the sample material from a list of candidates. BothdiffractGUIandringGUIemploy methods of computer vision for a fast, robust and accurate analysis. Thirdly,cellVieweris an intuitive visualization tool which is also helpful for crystallographic calculations or educational purposes.diffractGUIandcellViewercan be used together during a transmission electron microscopy session to determine the sample holder tilts required to reach a desired zone axis. All the tools offer a graphical user interface. The toolbox is distributed as a standalone application, so it can be installed on the microscope computer and launched directly fromDigitalMicrograph(Gatan Inc.).

Source: Journal of Applied Crystallography

DOI: 10.1107/s1600576715017252

Authors: Miloslav Klinger, Aleš Jäger

Institution: FZU - Institute of Physics of the Czech Academy of Sciences

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $5,110

Funder: Grantová Agentura České Republiky