A morphologic and immunohistochemical analysis of gastric adenocarcinoma with regard to the presence of E-cadherin and localisation of β-catenin staining
Year: 2014
Type: dissertation
Study design: Observational Study
Abstract: Introduction: Surgical resections for gastric carcinoma are a commonly encountered histopathologic specimen.Despite this, gastric carcinoma in South Africa and particularly within the Western Cape Province has not been well documented.Aims: To interrogate the WNT signalling pathway using selected protein expression by immunohistochemistry and correlating these with tumour subtypes. To assess Her2/neu expression and correlate this with morphologic subtypesObjectives: To determine aberrations in the expression of β-catenin, E-cadherin (extracellular and cytoplasmic domains) and DVL1 in gastric carcinoma subtypes.Additionally, to determine Her2/neu overexpression by immunohistochemistry and correlate this with morphologic subtype.Materials and methods: 97 gastric adenocarcinoma resection cases were retrieved and stained with antibodies against β-catenin, DVL1, E-cadherin (extracellular domain), E-cadherin (cytoplasmic domain) and Her2/neu.Results were considered statistically significant if P < 0.05.Results: All 97 cases were confirmed as gastric adenocarcinomas, with 39 (40%) intestinal type, 51 (53%) diffuse type and 7 (7%) mixed type tumours identified respectively.Patient ages ranged from 18 to 84 years.Her2/neu was overexpressed in 12 (12%) cases, with 9 of these cases showing intestinal-type morphology (P = 0.0174).Abnormal β-catenin localisation occurred in 14 (14%) cases.Aberrant E-cadherin (extracellular domain) localisation or absent staining was seen in 36 (37%) cases, with a significant proportion demonstrating diffuse type morphology (P <0.001).Abnormal E-cadherin (cytoplasmic domain) localisation or absent staining was seen in 7 (7%) cases, with a significant 6 of these 7 cases showing diffuse-type morphology (P = 0.0231).Eleven (11%) cases were seen in patients younger than 40 years, with 9 of these cases showing intestinal morphology and a significant 7 of the 11 cases showing aberrant Ecadherin (extracellular domain) localisation (P = 0.025).Her2/neu -Human epidermal growth factor receptor gene 2 HNPCC -Hereditary non-polyposis colorectal carcinoma IL-1β -interleukin 1 beta Int1 -Murine mammary tumour oncogenic integration site gene LRP -Low density lipoprotein receptor related protein MMed -Master of Medicine NHLS -National Health Laboratory Service NRF -National Research Fund NFAT -Nuclear factor associated with T cells Obj mag -Objective magnification PBS -Phosphate buffered saline TCF/LEF -T cell factor/lymphoid enhancer factor TNF-α -Tumour necrosis factor alpha SEER -Surveillance, Epidemiology and End Results SOX9 -Sex determining region Y box 9 SRFP -Secreted frizzled-related proteins UCT -University of Cape Town Vac A -Vacuolating gene A Wg -wingless
Author: Riyaadh Roberts
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2201455405