Year: 1973
Type: article
Abstract: Glutaraldehyde is well known for its ability to react with proteins and to produce insoluble cross-linked aggregates. In contrast with this situation, conditions are described here which yield covalently linked soluble protein oligomers. The procedure is applicable to a wide range of proteins, and by slight variation in the reaction conditions, soluble polymers in the molecular weight range 3x10(4)-2x10(7) were produced. The products are valuable as molecular-weight markers, e.g. in sodium dodecyl sulphate-polyacrylamide-gel electrophoresis. The inherent similarities of these oligomers make them superior to commercial molecular-weight protein markers, which may have marked differences in composition and charge.
Source: Biochemical Journal
DOI: 10.1042/bj1350867
Author: John W. Payne
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2201894882