Die Bedeutung der Nitrit-Reduktasefunktion von Myoglobin im myokardialen Ischämie/Reperfusionsschaden
Year: 2011
Type: dissertation
Abstract: Nitrite represents an endocrine bioavailable storage pool of nitric oxide (NO• ) that is bioactivated along the physiological oxygen gradient to regulate a number of vascular and cellular responses. Nitrite is present at concentrations at high concentrations of 1–20 µM in the myocardium. In vivo and ex vivo myoglobin showed under hypoxia the ability to reduce nitrite to NO•. In addition a cytoprotective Effect of Nitrite in myocardial ischemia and reperfusion has been shown. The mechanisem behind this protective effect remains unknown. The goals of this work have been: 1) to charectarise the effect of nitrite during ischemia and reperfusion in isolated mouse hearts, 2) to research the importents of the nitrite reductase function of myoglobin on the ischemia and reperfusion damage, 3) discover the biochemical fundamentals of the nitrite mediated cytoprotection, 4) clarify the the pathophysiological relevance of the results using an in vivo modell.The results of this research can be summarized as:1. Exogenes nitrite is dose related able to reduce the mycardial ischemia reperfusion damage in mouse hearts and improves the left ventricular function.2. The nitrite reductase activity of myoglobin mediates the observed cytoprotective effect of exogenes nitrite and the impproved left ventricular function. Hearts of myoglobin deficient mice showed no change in infarct size and function after aplication of nitrite.3. The protective effect of nitrite.
Source: RWTH Publications (RWTH Aachen)
Authors: Joel Schmitz, Tienush Rassaf
Language: German
Subfield: Cardiology and Cardiovascular Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2204123203