Work

Rectification of artificial molecular recombination with the use of high fidelity enzyme in the amplification of 16S rDNA sequences from Stool sample

Year: 2012

Type: article

Abstract: Reliance on routinely used taq polymerases for amplification may generate spurious sequences, especially in metagenomic studies utilizing complex mixtures of various DNA templates. Use of high fidelity enzymes and verification of the sequences using various software tools before submission to the databases ensures better quality and confidence.

Source: DOAJ (DOAJ: Directory of Open Access Journals)

Author: Vijay Nema

Institution: National AIDS Research Institute

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2207088212

FWCI: 0.105

Cites:

Cited by: 3

Related to: 10