Year: 2006
Type: article
Abstract: Previous work regarding anti-pneumococcal polysaccharide (PPS) variable (V) gene usage has used B cells isolated 7 days post-vaccination or 6 weeks post-vaccination. We hypothesized that V region gene usage of anti-PPS antibodies differs between B cells isolated 5-10 days and 4-6 weeks post-vaccination. Differential V gene usage between B cells isolated 5-10 days and 4-6 weeks post-vaccination was observed at the individual and population level. Serum analysis revealed that V regions isolated from circulating B cells at 5-10 days most closely reflects serum antibody present 5-10 days and 4-6 weeks post-vaccination. These results suggest that analysis of the physiologically relevant antiPPS VH/VL repertoire should be performed on PPS-specific circulating B cells isolated between 5-10 days post-vaccination.
Author: Brqadley James Rabquer
Institution: University of Toledo
Language: English
Topic: T-cell and B-cell Immunology
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
Funder: University of Toledo
OpenAlex ID: W2208363585