TNF-α and IL-10 Modulate CK and CK-MB Activities in Naturally Infected Dogs by Leishmania infantum
Year: 2014
Type: article
Abstract: "Background: Canine visceral leishmaniasis (CVL) is a multisystem inflammatory disease caused by the Leishmania genus protozoa, characterized by increased production of pro-inflammatory mediators. The parasite presence has been reported in various organs and tissues, such as bone marrow, lymph nodes, spleen, liver and skin, in addition the cardiac muscle. Cardiac muscle lesions provide the substances release, among which we can mention the creatine kinase (CK), its MB subunit (CK-MB) and troponin. The changes that cause these injuries depend not only of the parasite presence, but also the increased concentrations of proinflammatory cytokines. Although most studies focus on the proinflammatory cytokines action, regulatory cytokines of the inflammatory process has gained evidence in the immune response to the Leishmania infantum and can be directly associated with the cardiac damage pathogenesis. Therefore, this study aimed to evaluate the TNF-α and IL-10 serum levels in naturally infected dogs by L. infantum and relates them to cardiac biomarkers, in the different clinical forms of disease. Materials, Methods & Results: A total of 30 adult dogs were used, being 10 negative (ND) and 20 positive for CVL. All seropositive animals were subjected to clinical examination, observing the presence of characteristic clinical signs of disease, being divided into two groups: asymptomatic (AD, n = 10) and symptomatic (SD, n = 10) dogs. Blood samples from all animals were collected to obtain serum for subsequent measurement of TNF-α, IL-10, CK and CK-MB. Data were analyzed by Kruskal-Wallys test, followed by Dunn’s test. The correlation and influence of TNF-α, IL-10 and IL-10/ TNF-α on CK, CK-MB and CK-MB/CK, were determined by Spearman correlation test and linear regression. To evaluate the relationship between the clinical signs onset and the TNF-α, IL-10, IL-10/TNF-α, CK, CK-MB and CK-MB/CK serum levels, the unpaired t-test was used. TNF-α and IL-10, in the different groups, showed no significant differences. Already IL-10/TNF-α ratio was significantly increased in AD, when compared to ND. CK was significantly increased in infected animals, when compared to ND. Already the CK-MB was significantly increased in SD, when compared to ND. CK-MB/ CK ratio, in the different groups, showed no significant differences. TNF-α and IL-10 showed a moderate correlation and weak negative and positive influence on CK and CK-MB, in AD and SD. The IL-10/TNF-α ratio presented a weak correlation and moderate negative influence in AD, and moderate correlation and strong positive influence, in SD, on CK and CK-MB. It was also observed weak and moderate correlations, besides the weak and strong positive influences, of the IL-10/TNF-α ratio on CK-MB/CK ratio, in AD and SD dogs. Discussion: The elevation of CK and CK-MB serum levels, in asymptomatic and symptomatic animals, is directly related to the impairment of skeletal muscles, due to the increased production of proinflammatory cy"
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W2210264355