Year: 2005
Type: dissertation
Abstract: In this work we studied various aspects of DNA repair in plants, focusing mainly on point mutation repair and its interconnection with double-strand break repair.We were using transgenic point mutation and recombination substrates as a primary tool in our experiments.We have compared two transgenic homologous recombination assays (J3glucuronidase-and luciferase-based), analyzed the sensitivity of DNA repair machinery to ultraviolet radiation and assessed the involvement of AtKu80, Atm and AtXpd repair genes in point mutation repair.Our study revealed the following: the luciferase-based recombination assay is more sensitive then ft-glucuronidase-based; double-strand break repair machinery is sensitive to ultraviolet radiation, which results in increased point mutation formation; chosen DNA repair genes might be impaired in point mutation repair, however further experimentations are needed to confirm this.iii List of Abbreviations 6-4PP -6-4 pyrimidine pyrimidone 8-OHdG -8-hydroxydeoxyguanosine AP -apurinic/apyrimidinic BER -base excision repair CAK -component in associated complex CaMV -cauliflower mosaic virus CPD -cyclobutane pyrimidine dimmer DSB -double-strand break EMS -ethylmetane sulfonate EYFP -yellow fluorescent protein FYDR -fluorescent yellow direct repeat GGR -global genomic repair hGH -human growth hormone HR -homologous recombination IDL -insertion/deletion loop IR -ionizing radiation MMR -mismatch repair MMS -methylmetane sulfonate MS -Murashige and Skoog (medium) NAC -N-acetyl-L-acetyl NER -nucleotide excision repair NHEJ -non-homologous end joining PAM -polycyclic aromatic hydrocarbons PCB -polychlorinated biphenils PCD -programmed cell death PCR -polymerase chain reaction PPT -phosphinotricin RF -recombination frequency x ROS -reactive oxygen species RT-PCR -real-time PCR SCID -severe combined immunodeficiency SDSA -synthesis-dependent strand annealing SSA -single-strand annealing SSB -single-strand break TCR -transcription-coupled repair UV -ultraviolet (light) UV-A -ultraviolet light (400 -320 ran) UV-B -ultraviolet light (320 -290 nm) UV-C -ultraviolet light (290 -100 nm) xi
Author: Yaroslav Ilnytskyy
Language: English
Topic: DNA Repair Mechanisms
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2211024906