Automated Sub-micron Resolution Serial Block Face Imaging of Cancellous Bone Using Epifluorescence Microscopy
Year: 2008
Type: article
Abstract: Bone remodeling: The process of modifying bone microarchitecture by the coordinated efforts of osteoclast and osteoblast cells.Resorption cavity: A region on a bone surface created by the local resorptive activity of osteoclast cells.Resorption cavities are identified by a characteristic irregular surface texture (i.e.'scalloped surface') Serial block face imaging: An imaging technique which involves repeatedly removing the top of a specimen embedded in some medium and subsequently imaging the newly revealed cross-section Fluorescence microscopy: A microscopy imaging technique based on the light emitted from tissues autofluorescence or fluorescent markers as a result of incident excitation light Osteoporosis: A condition characterized by low bone mass and increased risk of fracture Bone mass: The quantity of bone based on bone mineral density.Bone mass is most commonly assessed by dual x-ray absorptiometry or quantitative computed tomography ix Osteoclast: Cells involved in the resorption (removal) of bone Osteoblast: Cells involved in the deposition (formation) of bone Stereology: A method of interpreting 2D measures in terms of 3D morphology Segmentation: Automatic or manual classification of image pixels as a specific tissue, feature, or signal type (e.g.bone tissue and background) Classification: See segmentation Voxel: An object's volumetric discretization stored digitally Dichroic mirror: A mirror designed to reflect or transmit specific wavelengths of light Finite element modeling (FEM): A numerical method of solving differential equations to achieve stress and strain distributions throughout a structure discretized into finite parts Kernel: A mathematical operator applied uniformly and repeatedly over a domain Point-spread-function: A three dimensional mathematical representation of optical blurring due to lens aberrations x Fiducial/fiduciary marker: A feature acting as a point of reference intended for alignment (registration) of images containing an object of irregular structure and/or unknown orientation Micro computed tomography: A non-invasive imaging technique utilizing x-ray radiation to collect high resolution three dimensional images of radiolucent specimens.
Author: Craig R. Slyfield
Institution: Case Western Reserve University
Language: English
Subfield: Orthopedics and Sports Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2211557804